Fluorescence spectroscopy is very important for studying protein structure and conformation changes.The concept and principle of fluorescence spectroscopy are introduced at first,then the application of studying fluorescence spectroscopy on protein is explained.
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[1]陶慰孙,姜涌明.蛋白质分子基础[M].北京:高等教育出版社,1995.
[2]Jiang Guohua,Wei Qun,Function and structure of N-termianl andC-termiandl domains of calcineurin B subunit[J].Biol Chem,2003,384(9):1299-303.
[3]王炜,廖国宁,季金林,等.荧光微量检测细胞内DNA与蛋白质含量[J].1999,28(3):267-272.
[4]陈国珍.荧光分析法[M].北京:科技出版社,1990.
[5]Lakowicz J R.Principles of fluorescence spectroscopy[M].NewYork:Plenum Press,1983.
[6]王峰,黄薇,唐波,等.蒂巴因与牛血清白蛋白的相互作用[J].分析化学研究简报,2006(34):239-242.
[7]谢光,刘芯韵,郑芸,等.茶碱与胃蛋白酶相互作用的光谱性质研究[J].天然产物研究与开发,2006(18):558-561.
[8]Souza E S,Hirata I Y,Juliano L,et al.End-to-end distance distribu-tion in bradykinin observed by Forster resonance energy transfer[J].Biochim Biophys Acta,2000,1474(2):251-261.
[9]loyd L.synchronized excitation of fluorecence emission spectra[J].Nature,1971(231):64-65.
[10]Inman E L,Winefordner J D,James Jr.Constrant energy syn-chronous flaorecence for analysis pdynuclear aromatic hydrocarbonmixtures[J].Anal Chem,1982(54):2018-2023.
[11]Miller J N.Recent advances in molecular luminescence analysis[J].Proc Anal Div Chem Soc,1979(16):203-209.
[12]黄贤智,许金钩,李耀群,等.同步荧光法同时测定色氨酸、酪氨酸和苯丙氨酸[J].分析化学,1987(15):199-202.
[13]BaeyensW R G,Keukeleire D D,Korkdis K.Luminescence tech-niques in chemical and biochemical analysis[M].New York:Marcel Dekker,1991:381-419.
Basic Information:
DOI:10.16791/j.cnki.sjg.2010.02.011
China Classification Code:Q51
Citation Information:
[1]尹燕霞,向本琼,佟丽.荧光光谱法在蛋白质研究中的应用[J].实验技术与管理,2010,27(02):33-36+40.DOI:10.16791/j.cnki.sjg.2010.02.011.
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“生物化学大实验校级精品课”建设项目;; “生命科学创新人才实验教学体系建设与改革”教改项目(127016)